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. 2023 May 5;14:2612. doi: 10.1038/s41467-023-38016-4

Fig. 3. Transcription factors expressed in putative specialized neoblast populations are required for the differentiation of major cell types.

Fig. 3

a UMAP projection of transcription factors expressed in putative differentiation trajectories from URD analysis. All transcription factors identified were found to be expressed in their corresponding differentiated cell types and/or putative specialized neoblast populations. b Double FISH of the candidate transcription factors with the stem cell marker piwi-1 during regeneration for putative endodermal-like/digestive, neural, muscle, and epidermal specialized neoblasts. Co-expression of piwi-1 and candidate transcription factors (denoted by white arrowheads) was detected at 72 hours post amputation (hpa) for putative endodermal/digestive, neural, and muscle specialized neoblasts while putative epidermal specialized neoblasts were detected at 48 hpa. Scale bars 10 µm. c RNAi of putative specialized neoblast transcription factor leads to loss of differentiated cell type expression during regeneration. Regenerating tail fragments are shown 7 days post amputation (dpa) and corresponding head fragments are in Fig. S3e. Scale bars 100 µm. Schematic of regenerating Hofstenia tail in (c) reprinted from Cell Reports, Volume 32, Issue 9, Ramirez, A.N., Loubet-Senear, K., and Srivastava, M., A Regulatory Program for Initiation of Wnt Signaling during Posterior Regeneration, 108098, Copyright (2020), with permission from Elsevier.

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