Skip to main content
. 2023 Apr 24;3(4):100456. doi: 10.1016/j.crmeth.2023.100456
Antibodies (primaries) Species Concentration Source Identifier
POU5F1 (ICC) Mouse 1:200 Santa Cruz Cat# SC-5279; RRID:AB_628051
NANOG (ICC) Goat 1:200 R&D Cat# AF1997; RRID:AB_355097
SOX2 (ICC) Rabbit 1:200 Millipore Cat# AB5603; RRID:AB_2286686
POU5F1-PecCP5.5 (FC)
Isotype-PerCP5.5
Mouse 20 μL per 1x106 cells BD-Biosciences Cat# 560794; RRID:AB_1937313
Cat# 552834; RRID:AB_394484
NANOG-647 (FC)
Isotype-647 (FC)
Mouse 5 μL per 1x106 cells BD-Biosciences Cat# 561300; RRID:AB_10611718
Cat# 557714; RRID:AB_396823
SOX2-488 (FC)
Isotype-488 (FC)
Mouse 2.5 μL per 1x106 cells BD-Biosciences Cat# 561593; RRID:AB_10894382
Cat# 557721; RRID:AB_396830
SSEA1-PE (FC)
Isotype-PE (FC)
Mouse 20 μL per 1x106 cells BD-Biosciences Cat# 560886; RRID:AB_10584320
Cat# 555584; RRID:AB_395960
SSEA4-647 (FC)
Isotype-647 (FC)
Mouse 5 μL per 1x106 cells BD-Biosciences Cat# 563119; RRID:AB_2738015
Cat# 563274; RRID: AB_2869481
TNNT2 (ICC) Mouse 1:200 Invitrogen Cat# MA5-12960; RRID:AB_11000742
TNNT2-PE (FC)
Isotype-PE (FC)
Mouse 5 μL per 1x106 cells BD Cat# 564767; RRID:AB_2738939
Cat# 554680; RRID:AB_395506
MYL2 (ICC/FC) Rabbit 1:100/1:100 for 5x105 cells Proteintech Cat# 10906-1-AP; RRID:AB_2147453
HAND1 (ICC/FC) Goat 1:100/1:100 for 5x105 cells R&D Cat# AF3168; RRID:AB_2115853
NR2F2 (ICC) Mouse 1:150 R&D Cat# PP-H7147-00; RRID:AB_2155627
TNNI3 (ICC) Goat 1:200 Abcam Cat# Ab56357; RRID:AB_880622
NKX2.5 (ICC) Rabbit 1:150 Santa Cruz Cat# SC-14033; RRID:AB_650281
NKX2.5 (ICC) Goat 1:100 Santa Cruz Cat# SC-8697; RRID:AB_650280
GATA4 (ICC) Rabbit 1:150 Cell signaling Cat# 36966; RRID:AB_2799108
MSX1 (ICC) Goat 1:200 R&D Cat# AF5045; RRID:AB_2148804
TBXT, BRA (ICC) Goat 1:150 R&D Cat# AF2085; RRID:AB_2200235
EOMES (ICC) Rabbit 1:200 Abcam Cat# Ab23345; RRID:AB_778267
ISL1 (ICC) Goat 1:200 R&D Cat# AF1837; RRID:AB_2126324
ISL1 (ICC) Rabbit 1:50 Abcam Cat# Ab20670; RRID:AB_881306
TBX5 (ICC) Rabbit 1:50 Thermo Fisher Scientific Cat# 42–6500; RRID:AB_2533533
FOXA2 (ICC) Rabbit 1:150 Cell Signaling Cat# 8186; RRID:AB_10891055
CTNNB1 (ICC/WB) Rabbit 1:200/1:1000 Sigma Cat# C2206; RRID:AB_476831
ACTA2 (ICC) Mouse 1:200 cline 1A4; Sigma Cat# A2547; RRID:AB_476701
TCAP (ICC) Mouse 1:20 Santa Cruz Cat# SC-25327; RRID:AB_628340
MYOM1 (ICC) Mouse 1:100 clone B4;107 N/A
MYOM2 (ICC) Mouse 1:5 108 N/A
DES (ICC) Mouse 1:200 Dako Cat# M0760; RRID:AB_2335684
ACTN2 (ICC) Rabbit 1:500 Abcam Cat# Ab68167, RRID:AB_11157538
Ki67 (ICC) Rabbit 1:200 Abcam Cat# Ab15580; RRID:AB_443209
RYR2 (ICC) Mouse 1:200 Abcam Cat# Ab2868; RRID:AB_2183051
ACTN2 (ICC) Mouse 1:400 Sigma Aldrich Cat# A7811; RRID:AB_476766
GFP (ICC) Rabbit 1:1000 Abcam Cat# Ab6556; RRID:AB_305564
IK1(WB) Mouse 1:100 Santa Cruz Cat# SC-365265; RRID:AB_10841432
PLN (WB) Rabbit 1:250 Abcam Cat# Ab92697, RRID:AB_10585584
ATPA2 (WB) Mouse 1:1000 Santa Cruz Cat# SC-376235; RRID:AB_10989947
CTNNB1 (WB) Rabbit 1:500 Cell Signaling Cat# 9562S; RRID:AB_331149
TUBA1A/B (WB) Mouse 1:2000 Sigma Cat# T5168; RRID:AB_477579
Oxphos kit (WB) Mouse 1:2000 Abcam Cat# ab110411; RRID:AB_2756818
Antibodies (secondaries) Species Concentration Source Identifier
Anti-Rabbit-Alexa 488 Donkey 1:500 Thermo Fisher Scientific Cat# A-21206; RRID:AB_2535792
Anti-Goat-Alexa 647 Donkey 1:500 Thermo Fisher Scientific Cat# A-21447; RRID:AB_141844
Anti-Mouse-Alexa 594 Donkey 1:500 Thermo Fisher Scientific Cat# A-21203; RRID:AB_141633
Anti-Rabbit-Alexa 594 Donkey 1:500 Thermo Fisher Scientific Cat# A-21207; RRID:AB_141637
Anti-Goat-Alexa 594 Donkey 1:500 Thermo Fisher Scientific Cat# A-11058; RRID:AB_142540
Anti-Mouse-Alexa 488 Donkey 1:500 Thermo Fisher Scientific Cat# A-21202; RRID:AB_141607
Anti-Mouse-Alexa 647 Donkey 1:500 Thermo Fisher Scientific Cat# A-31571; RRID:AB_162542
Anti-Goat-Alexa 488 Donkey 1:500 Thermo Fisher Scientific Cat# A-11055; RRID:AB_2534102
Anti-Mouse-HRP Goat 1:2000 Bio-Rad Cat# 1706516; RRID:AB_2921252
Anti-Rabbit-HRP Goat 1:2000 Bio-Rad Cat# 1706515; RRID:AB_2617112
Anti-Mouse-IRDye 800CW Goat 1:10000 LI-COR Cat# 926–32210; RRID:AB_621842
Anti-Rabbit-IRDye 689LT Goat 1:10000 LI-COR Cat# 926–68021; RRID:AB_10706309