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. 2023 May 5;118(1):17. doi: 10.1007/s00395-023-00981-8

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Contribution of Cd34-lineage mesenchymal cells in I/R injury. A UMAP plot showing 7 color-coded subclusters from the mesenchymal (Mesen) cell population in I/R injury, n = 14,125 cells. B Total-cell and tdTomato⁺ (tdT⁺) cell datasets from all time points (sham, I/R 1w, I/R 2w) were integrated, mesenchymal cell population were subclustered for deep investigation. Violin plot showing expression of selected marker genes across all mesenchymal cell subclusters, with putative cell identities annotated below. C Proportion of tdT⁺ cells in each mesenchymal cell subcluster. D, E SCORPIUS (D) and Monocle (E) trajectory analyses showing the relationship among tdTomato⁺ mesenchymal cell subclusters and potential differentiation directions over pseudotime. The branch point 2 selected for downstream analyses is indicated by the red circle. F Heatmap of the significantly changed genes (P < 0.01) discovered by the BEAM function from monocle in the branch point 2 in (E), with selected upregulated genes listed at different cell states (prebranch, cell fate 1 and 2). G GOBP analysis of significantly changed genes (P < 0.01) in different cell fates discovered by the BEAM function from Monocle in the first branch point (branch point 2) from the trajectory in (E). H Representative images of Cre/TDT ventricles staining tdT with Sca-1, Periostin, Ifitm1 and Ifitm2 in border zones, with magnification of the boxed region, arrows indicated co-staining cells. Scale bars, 100 μm, and 20 μm in magnification. IFN, interferon stimulated. Mesen, mesenchymal cell. tdT tdTomato