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. 2023 May 5;118(1):17. doi: 10.1007/s00395-023-00981-8

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — Contribution of Cd34-lineage monocytes/macrophages in I/R injury. A UMAP plot showing 9 color-coded subclusters from the monocytes/macrophages population in I/R injury, n = 4579 cells. Dot plot shown on the right indicating the expression of selected marker genes across all subclusters, with putative cell identities annotated in the middle. B Proportion of tdT⁺ cells in each monocyte/macrophage subcluster. Gene set enrichment analysis (GSEA) shown on the right indicated significant pathways enriched in recruited subclusters (monocytes, Spp1⁺ macrophages). C Sketch of the experimental design for generating chimeric C57BL6 mice with bone marrow cells transplanted from Cre/TDT mice. Chimeric mice were subjected for I/R injury and heart samples were harvested for further investigation. D Representative images of chimeric C57BL6 murine ventricles staining tdT with vimentin, CD31 and CD68 in border zones, with magnification of the boxed region, arrows indicated co-staining cells. Scale bars, 100 μm, and 20 μm in magnification. Quantification of the percentages of vimentin⁺/CD31⁺/CD68⁺ cells in BM-derived tdTomato⁺ cells by immunofluorescence was shown on the right panel. N = 4 mice, ***P < 0.001, by one-way ANNOVA test. BM bone marrow, ISG interferon stimulated, tdT tdTomato