Fig. 4.

Weakly active and developmentally regulated genes are susceptible to downregulation in H3K4me3-deficient beta cells. (a–l) Average enrichment profiles and quantification of H3K4me3 (a, b), H3K4me1 (c, d), H3K27ac (e, f), H3K27me3 (g, h), DNAme (i, j) and G/C nucleotide content (k, l) in Dpy30-WT cells for genes downregulated, stably expressed or upregulated by Dpy30-KO. (m) Box and whisker plot showing RNA expression in Dpy30-WT cells for genes downregulated, stably expressed or upregulated by Dpy30-KO. (n) Running enrichment plot for imprinted genes ([83]), genes up- or downregulated during mouse beta cell maturation (adults vs postnatal day 10, [63]) and mature beta cell transcription factor genes. P values were calculated using Wilcoxon rank-sum (b–m) or permutation (n) tests with Benjamini–Hochberg correction. *p<0.05, **p<0.01, ***p<0.001. NES, normalised enrichment score; ns, not significant; TES, transcription end site