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. 2023 Mar 13;66(6):1097–1115. doi: 10.1007/s00125-023-05896-6

Fig. 5.

Fig. 5

H3K4me3 deficiency leads to a less active and more repressed epigenome in mature beta cells. (a) Genome browser view of H3K4me3, H3K4me1, H3K27ac and H3K27me3 in Dpy30-WT and Dpy30-KO cells showing preservation of the megabase-scale organisation of H3K27me3-positive Polycomb-repressed compartments. (b, c) Scatterplots of H3K27ac (b) and H3K27me3 (c) enrichment in 10 kb bins spanning the genome in Dpy30-KO vs Dpy30-WT cells. (d, e) Average enrichment profiles of H3K27ac (d) and H3K27me3 (e) at the TSS of all expressed genes in Dpy30-WT and Dpy30-KO cells. (f, g) Box and whisker plots showing the log2(fold change) in H3K27ac (f) and H3K27me3 (g) enrichment in the TSS of downregulated, stable and upregulated genes in Dpy30-KO vs Dpy30-WT cells. (h) Genome browser view of a downregulated gene, Rab34, showing loss of H3K4me3, H3K4me1, H3K27ac and RNA and accumulation of H3K27me3 in Dpy30-KO cells. P values were calculated using Wilcoxon rank-sum tests with Benjamini–Hochberg correction. ***p<0.001