Fig. 1. Monomer and protein complex structure prediction metrics distinguish previously designed binders from non-binders.
a For binder design to be successful, the designed sequence must fold to the designed binder monomer structure (left), and this structure must form the designed interface with the target protein (right). b, c Design failure modes. b Type-1 Failures. The designed sequence does not fold to the designed monomer structure. c Type-2 Failures. The designed sequence folds to the designed monomer structure but does not form the designed interface. d, e The retrospective experimental success rate (YSD SC50 < 4 μM) for the top 1% of designs selected according to different monomer (d) or protein complex (e) based metrics over 10 targets from Cao et al. Source data are provided as a Source Data file.