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. 2023 Apr 21;150(8):dev201273. doi: 10.1242/dev.201273

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© 2023. Published by The Company of Biologists Ltd

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed.

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Fig. 6. — Ror2 domain analysis reveals differential requirements for cartilage stacking. (A) Schematic of ror2 transgenic constructs. A sox10 enhancer element drives expression of Ror2-SuperFolderGFP (sfGFP) fusions in a Tol2 vector. Constructs include full-length Ror2-sfGFP (Ror2FL), Ror2K509R-sfGFP (KD), Ror2ΔCRD-sfGFP (ΔCRD), Ror2ΔPRD-sfGFP (ΔPRD), Ror2ΔC-sfGFP (ΔC) and full-length Ror1 (Ror1FL). Asterisk indicates the location of the K509R mutation. (B,C) Confocal images of fluorescent cartilages at 3 dpf in WT (B) and ror2^−/− (C) in a Tg(sox10:lyn-tdTomato) background. (D-O) Representative rescue construct mosaic transgenic cartilages in grayscale showing sox10:lyn-tdTomato only (D,F,H,J,L,N) or GFP only (E,G,I,K,M,O). White arrowheads indicate the symplectic cartilage. Panels B-O are z-projections. (P) Quantification of symplectic cartilage length, color-coded according to magnitude. **P<0.01; ***P<0.001 (Kruskal–Wallis test with post-hoc Dunn's test and Bonferroni correction). ns, not significant. Box plot shows the median (middle bar) and first to third interquartile ranges (boxes); whiskers indicate 1.5× the interquartile ranges; dots indicate data points. CRD, cysteine-rich domain; Ig, immunoglobulin-like domain; Kr, Kringle domain; PRD, proline-rich domain; ST1, serine-threonine domain 1; ST2, serine-threonine domain 2; TM, transmembrane domain. Scale bar: 20 μm for B-O.