Fig. 2.

Cellular uptake and subcellular distribution of SIL@T. A) Diagram of the intracellular drug release. B) Flow cytometry results of micellar uptake by 4T1-Br-Luc cells (1 h after formulation administration), FITC: coumarin-6. C) Quantitative results of SIL@T uptake by 4T1-Br-Luc cells after pretreatment with inhibitors of different uptake pathways (1 h after formulation administration). D) Uptake of dual fluorescently labeled SIL@T (BODIOY-labeled polymer, coumarin-6 mimic encapsulated SIL) by 4T1-Br-Luc cells at 0.5 h (left) and 1 h (right). The pixel intensity maps are plotted along the red line. Blue: nucleus; green: coumarin-6; white-grey: endosome/lysosome; red: micelles. Scale bars: 20 μm.