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. Author manuscript; available in PMC: 2023 Jul 1.
Published in final edited form as: Exp Eye Res. 2022 May 18;220:109112. doi: 10.1016/j.exer.2022.109112

Fig, 3: Freeze injury assay induces a circular decellularized wound on PA substrata.

Fig, 3:

(A) Schematic overview of freeze injury experiment. A liquid nitrogen-cooled rod is used to create a circular freeze injury within a confluent monolayer of corneal keratocytes. (B) Representative phase contrast images of a freeze injury on a stiff (10 kPa) PA substratum after 0 and 96 hr of time-lapse culture. Note that keratocytes have migrated into the decellularized region. Scale bar, 200 μm.