Figure 7. Silencing PCG glutamatergic and GABAergic projections to specific targets attenuates aversion and reward-related behavior, respectively.

(A) Optogenetic silencing (bilaterally) of PCG glutamatergic axon terminals in VTA.

(B) Summary of percentage time spent in the stimulation chamber in eNpHR (n = 5 for each subgroup) and eYFP (n = 5 for each subgroup) groups under different conditions. *P < 0.05, one-way ANOVA.

(C) Summary of increase in average speed in the stimulation chamber. P > 0.05, one-way ANOVA.

(D) Normalized Δ pupil size in noise alone and noise plus LED conditions in eNpHR (n = 5) and eYFP (n = 5) groups. * P < 0.05, paired t-test.

(E) Optogenetic silencing (bilaterally) of PCG glutamatergic axon terminals in LHA.

(F) Summary of percentage time spent in the stimulation chamber in the eNpHR (n = 5 for each subgroup) and eYFP (n = 5 for each subgroup) groups. *P < 0.05, one-way ANOVA.

(G) Summary of increase in average speed in the stimulation chamber in eNpHR (n = 5 for each subgroup) and eYFP (n = 5 for each subgroup) groups. **P < 0.01, one-way ANOVA.

(H) Normalized Δ pupil size in noise alone and noise plus LED conditions in eNpHR (n = 5) and eYFP (n = 5) groups. **P < 0.01, paired t-test.

(I) Optogenetic silencing of PCG GABAergic axon terminals in the LHA and VTA separately.

(J) Summary of sucrose preference without and with LED illumination in eNpHR (n = 6) and eYFP (n = 5) groups. *P < 0.05, paired t-test.

(K) Normalized Δ pupil size in sucrose alone and sucrose plus LED conditions in eNpHR (n = 6) and eYFP (n = 5) groups. **P < 0.01, paired t-test.

(L) Summary of sucrose preference without and with LED illumination in eNpHR (n = 5) and eYFP (n = 5) groups. *P < 0.05, paired t-test.

(M) Normalized Δ pupil size in sucrose alone and sucrose plus LED conditions in eNpHR (n = 5) and eYFP (n = 5) groups. **P < 0.01, paired t-test.

Error Bar = SD in all plots.