KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Fluorescent Nissl Stain | Invitrogen | RRID: AB_2572212 |
| RNAscope Mm-Slc17a6 | ACDbio | Cat# 319171-C2 |
| RNAscope Mm-Slc32a1 | ACDbio | Cat# 319191-C3 |
| Bacterial and Virus Strains | ||
| AAV1-CAG-FLEX-GFP-WPRE | A gift from Hongkui Zeng94 | Addgene plasmid # 51502 |
| AAV1-EF1a-DIO-hChR2-eYFP | A gift from Karl Deisseroth | Addgene plasmid # 20298 |
| AAV1-EF1a-DIO-eYFP | A gift from Karl Deisseroth | Addgene plasmid # 27056 |
| AAV1-CAG-FLEX-ArchT-GFP | A gift from Edward Boyden95 | Addgene plasmid # 29777 |
| pAAV-hSyn-hChR2 (H134R)-EYFP | A gift from Karl Deisseroth | Addgene plasmid # 26973 |
| pAAV-TREtight-mTagBFP2-B19G | A gift from Ian Wickersham60 | Addgene plasmid # 100799 |
| pAAV-Syn-FLEX-splitTVA-EGFP-tTA | A gift from Ian Wickersham60 | Addgene plasmid # 100798 |
| RVΔG-4mCherry (EnVA) | A gift from Ian Wickersham | N/A |
| pAAV-Ef1a-DIO-eNpHR-3.0-EYFP | A gift from Karl Deisseroth96 | Addgene plasmid # 26966 |
| AAV1-Syn-FLEX-GCamp6s-WPRE-SV40 | A gift from Douglas Kim & GENIE Project97 | Addgene plasmid # 100845 |
| Chemicals, peptides, and recombinant proteins | ||
| Kwik-Cast Sealant | WPI, Inc. | KWIK-CAST |
| Paraformaldehyde | Alfa Aesar OmniPur | 10194340 |
| NaCl | OmniPur | UI27FZEMS |
| KCl | Mallinckrodt | 7447-40-7 |
| NaHCO3 | EMD Chemicals | 48204847 |
| MgCl2 | J.T. Baker | 7791-18-6 |
| CaCl2 | EMD Chemicals | 41046444 |
| Glucose | Sigma | SLBC6575V |
| Sucrose | Millipore | D00168514 |
| Agarose | OmniPur | 3332C511 |
| DAPI | ACDbio | 323108 |
| Dil | Invitrogen | D282 |
| Muscimol,>98%; Tocris; 10mg | Fisher Scientific | 28910 |
| Experimental models: Organisms/strains | ||
| Mouse: C57BL/6J | The Jackson Laboratory | RRID: IMSR_JAX:000664 |
| Mouse: Ai14 | The Jackson Laboratory | RRID: IMSR_JAX:007914 |
| Mouse: VGluT2-ires-Cre mice | The Jackson Laboratory | RRID: IMSR_JAX: 016963 |
| Mouse: VGAT-ires-Cre mice | The Jackson Laboratory | RRID: IMSR_JAX: 016962 |
| Software and Algorithms | ||
| Data acquisition with Labview | LabVIEW | http://www.ni.com/en-us/shop/labview.html: RRID: SCR_014325 |
| Custom-written MATLAB code for analysis | MATLAB | http://www.mathworks.com/; RRID: SCR_001622 |
| Allen Reference Atlas | (Dong, 2008)98 | http://www.brainmap.org/; RRID: RRID: SCR_008848 |
| Offline sorter | Plexon | http://plexon.com/;RRID: RRID: SCR_000012 |
| Prism | GraphPad | https://www.graphpad.com/scientific-software/prism/; RRID:SCR_002798 |
| Custom-written python code for analysis | Python | https://www.python.org/; RRID:SCR_008394 |
| OpenCV library | OpenCV | https: opencv.org/;RRID: SCR_015526 |
| Fiji | NIH | https://fiji.sc/; RRID:SCR_002285 |
| Original code for analysis of behavioral data | https://doi.org/10.5281/zenodo.7604169 | https://doi.org/10.5281/zenodo.7604169 |
| Other | ||
| Free Field Speaker | TDT MF1 | N/A |
| Sound-Attenuation Booth | Gretch-Ken Industries | N/A |
| NI board for sound generation | National Instrument | PCl-6731 |
| Microvalve | Lee Co. | LFAA1209512H |
| Optrode | Neuronexus Technologies | A1x16-Poly2-5mm-50 s-177-OA16LP |