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. 2023 May 6;9:147. doi: 10.1038/s41420-023-01453-7

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — A–C. Cytotoxicity analysis of B7-H3 CAR-T cells against B7-H3 positive target cells by real-time cell assay. The data are represented as the mean cytotoxicity ± SEM. D–F Flow cytometry detected the final proportion of T cells co-cultured with PC3 (D), DU145 (E) or LNCaP (F), and calculated the percentage of killing. The data are represented as the mean cytotoxicity ± SEM. G, H Effect of tumor cells on the proliferation of B7-H3 CAR-T cells by FCM; flow cytometry was used to detect the fluorescence intensity of control T and B7-H3 CAR-T cells to reflect the proliferation of T cells after co-incubating CFSE-stained T cells with PC3 (G) or DU145 (H). I, J Effect of tumor cells on the cytokine release of B7-H3 CAR-T cells by FCM; T cells were subjected to membrane permeation, fixation, and antibody staining to detect the IFN-γ (I) and TNF-α (J) levels of control T and B7-H3 CAR-T cells under the stimulation of prostate cancer cells. These data are expressed as the mean percentage of cytokines ± SEM. **P < 0.05, ***P < 0.001.