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. 2023 May 6;9:147. doi: 10.1038/s41420-023-01453-7

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — A, B. The expression of B7-H3 protein on the surface of PC3(B7H3-) (A) and DU145(B7H3-) (B) monoclonal cells was detected by flow cytometry. C, D Western blot was utilized to confirm the expression of B7-H3 in PC3, PC3(B7H3-), DU145, and DU145(B7H3-) cells. E, F RTCA was used to verify the killing of control T and B7-H3 CAR-T cells to B7-H3 negative PC3(B7H3-) (E) and DU145(B7H3-) (F), and the right figure is the corresponding statistics. G, H The killing of PC3(B7H3-) (G) or DU145(B7H3-) (H) with B7-H3 CAR-T and control T cells was assessed via FCM. The killing effect of T cells on tumor cells was calculated by the mean ± SEM. I, J CFSE staining was used to detect the proliferation of control T and CAR-T cells co-incubated with PC3(B7H3-) (I) or DU145(B7H3-) (J). K, L The release of IFN-γ (K) and TNF-α (L) from T cells under the stimulation of B7-H3 negative tumor cells. These data are exhibited as the mean percentage of cytokines ± SEM. **P < 0.05.