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. 2022 Dec 8;51(8):4055–4063. doi: 10.1093/nar/gkac1152

Figure 1.

Figure 1.

Light-induced toehold clipping process. (A) Toehold-based DNA strand displacement process. Sequence identities are denoted by alphabets in italics. Arrows represent 3′ ends of strands. (B) Toehold-based strand displacement in a duplex with a photocleavable linker (PCL) at the toehold. (C) On UV exposure, the photocleavable linker is cleaved, removing the toehold and preventing strand displacement. (D) Chemical structure of the PCL incorporated in DNA strand and cleavage products after UV exposure. (E) Cleavage of ssDNA containing a PCL analyzed by denaturing PAGE. (F) Time series of photocleavage of PCL-containing DNA. (G) UV-induced toehold clipping in a DNA duplex. (H) Strand displacement in duplexes before and after toehold clipping. (I) Strand displacement efficiency in control duplex, PCL-TH duplex without UV exposure, and PCL-TH duplex on UV exposure resulting in toehold clipping.