Table 2.
Quantification of 7-deazaguanine modifications in pUC19 DNA treated with DpdABC under varying conditions
# | Conditions | dADG per 106 nt | dPreQ0 per 106 nt |
---|---|---|---|
1 | DpdAB | nda | 3593 ± 212b |
2 | DpdABC | 7844 ± 456 | 31 ± 3 |
3 | DpdC with preQ0-modified DNA | 9987 ± 536 | 238 ± 14 |
4 | DNA control (no enzyme) | nd | nd |
and, not detected; bmodifications per 106 nucleotides; values represent the mean ± deviation about the mean for two technical replicates of a single biological sample.