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Apparent KD values for ProXp-ala binding to A76 duplexPro variants as determined from EMSAs. Values were determined from at least three independent trials as explained in the Materials and Methods
| Variant | Apparent KD (μM) |
|---|---|
| A76 (WT) | 33 ± 11 |
| dA76 | 34 ± 5 |
| △A76 | 75 ± 12 |
| 3′p△A76 | 63 ± 11 |
| A76C | 87 ± 4 |
| A76G | 66 ± 11 |
| A76U | 106 ± 16 |
