Skip to main content
. 2023 Feb 16;51(8):3679–3695. doi: 10.1093/nar/gkad088

Figure 1.

Figure 1.

VirB triggers a change in electrophoretic mobility and topoisomer distribution of the PicsP-lacZ reporter plasmid. (A) Schematic of VirB-dependent PicsP-lacZ carried by the reporter plasmid, pHJW20, used throughout this study (not drawn to scale). Previously identified regulatory elements are indicated (48,49,92). Note, only primary TSS is shown (92). (B) Electrophoretic mobility of wild-type PicsP-lacZ reporter isolated from E. coli DH10B in the presence or absence of virB induction during growth. *, denotes position of the change in electrophoretic mobility of PicsP-lacZ. This gel was run in the absence of ethidium bromide and stained afterwards. C) Analysis of PicsP-lacZ topoisomer distribution of samples from (A) with an agarose gel containing chloroquine (2.5 μg ml−1) [R = relaxed and SC = supercoiled]. Quantification of key lanes (3 & 4) on gels shown in (B) and (C) is provided in Supplementary Figure S6A–D.