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. 2023 Mar 17;51(8):e47. doi: 10.1093/nar/gkad169

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© The Author(s) 2023. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 2. — SiT reveals isoform switches in the mouse olfactory bulb. (A) Data-driven annotation (left panel) of mouse olfactory bulb spatial regions through transcriptome-clustering of short-read data. Heatmap shows the expression of prominent marker genes for each region. (B) Exonic structure of the different Plp1 isoforms (mm10 genome build) detected by SiT. (C, D) Expression of Plp1 isoforms detected by SiT (C) and ISS (D). (E) Uniform Manifold Approximation and Projection (UMAP) representation of an external MOB single cell dataset (27). The dot plot on the right indicates Plp1 expression per cell type in the single cell dataset. (f) Spatial spot deconvolution of cell types with high/prominent Plp1 expression. Each dot corresponds to a pie graph indicating cell type composition in this spot (upper panel). Per spatial spot correlation observed between deconvolution score and Plp1 isoform expression (lower panel). Results show that Plp1 is predominantly expressed by olfactory ensheathing cells (OEC) in the olfactory nerve layer (ONL) and by myelinating-oligodendrocytes (MyOligo) in the granule cell layer (GCL).