TABLE 1.
MICs of macrolides against P. aeruginosa producing a macrolide-inactivating enzyme and properties of the enzyme
| Strain | MIC (μg/ml)a
|
Inactivation of oleandomycin by crude enzyme with the cofactor:
|
mphA primer PCR products | ||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| 14-MRb
|
AZM (15-MR) | 16-MR
|
|||||||||||||||
| OL | TAO | EM | CAM | RXM | JM | LM | MDM | MOM | RKM | SPM | TYL | ATP | GTP | Other | |||
| M397 | >1,600 | >800 | >1,600 | >800 | >800 | >1,600 | >400 | >800 | >200 | >200 | >400 | >1,600 | 800 | + | + | − | + |
| M398 | >1,600 | >800 | >1,600 | >800 | >800 | >1,600 | >400 | >800 | >200 | >200 | >400 | >1,600 | 800 | + | + | − | + |
| PAO2142Rpc | >1,600 | 800 | 100 | 100 | 200 | 25 | >400 | 800 | >200 | >200 | 400 | 1,600 | 800 | ||||
a
OL, oleandomycin; TAO, triacetyloleandomycin; EM, erythromycin; CAM, clarithromycin; RXM, roxithromycin; AZM, azithromycin; JM, josamycin; LM, leucomycin; MDM, midecamycin; MOM, miokamycin; RKM, rokitamycin; SPM, spiramycin; TYL, tylosin.
b
14-MR, 14-membered ring.
c
Recipient strain.