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. 2023 Apr 21;14:1176044. doi: 10.3389/fimmu.2023.1176044

Figure 3.

Figure 3

Evaluation of lung microbiota members for identification of anti-inflammatory and non-cytotoxic species in (3D) A549 (luciferase NF-κB-reporter) cells. (A) Isolated lung commensals at MOI 50 and MOI 12.5, with or without LPS, were screened for anti-inflammatory activity by a NF-κB luciferase assay. On the vertical axis, screened lung commensals are depicted, on the horizontal axis, the % NF-κB pathway activation compared to LPS-stimulated cells is shown. Anti-inflammatory effects were defined as;< 50% NF-κB pathway activation compared to LPS-stimulated cells as visualized by a green line, p< 0.05. Anti-inflammatory commensals are marked in red on the graph. N ≥ 3. (B) Isolated lung commensals at MOI 50 and MOI 12.5, with or without LPS, were screened for cytotoxicity by a LDH assay. On the vertical axis, screened lung commensals are depicted, on the horizontal axis, the % viability compared to cells cultured alone is shown. Cytotoxic effects were defined as;< 80% viability compared to cells cultured alone as visualized by a green line, p< 0.05. Cytotoxic commensals are marked in red on the graph. N ≥ 3.