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. 2023 Apr 21;14:1176044. doi: 10.3389/fimmu.2023.1176044

Figure 5.

Figure 5

Determination of the minimal effective MOI (mMOIIL-8) of commensal bacteria in (3D) A549 cells based on IL-8 secretion. Selected lung commensals at different MOI were cultured with LPS and the mMOIIL-8 was determined using an ELISA for IL-8 quantification, with (A) A. naeslundii, (B) G. haemolysans, (C) P. nigrescens, (D) R. mucilaginosa, (E) S. sanguinis. On the vertical axis % IL-8 production compared to LPS-stimulated cells is shown, on the horizontal axis the tested MOI is depicted. The mMOIIL-8 was defined as the lowest MOI with anti-inflammatory activity (i.e.< 50% IL-8 production compared to LPS-stimulated cells as visualized by a green line, p< 0.05). *: p< 0.05, n ≥ 3, n.t.: not tested. (F) Comparison of the minimal MOI for inhibition of NF-κB pathway activation and IL-8 production. On the vertical axis the minimal MOI is shown, on the horizontal axis the selected microbiota members are depicted. Anti-inflammatory activity was defined as:< 50% NF-κB pathway activation/IL-8 secretion compared to LPS-stimulated cells, p< 0.05.