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. 2023 Apr 21;14:1176044. doi: 10.3389/fimmu.2023.1176044

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Copyright © 2023 Goeteyn, Grassi, Van den Bossche, Rigauts, Vande Weygaerde, Van Braeckel, Maes, Bracke and Crabbé

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Anti-inflammatory activity of selected lung commensals with 1 mM H₂O₂ as pro-inflammatory stimulus in (3D) A549 cells based on IL-8 secretion. Selected lung commensals at their mMOI_IL-8, in presence of H₂O₂, were evaluated for anti-inflammatory activity using an ELISA for IL-8 quantification. On the vertical axis the tested species are shown. On the horizontal axis the % IL-8 production compared to H₂O₂-stimulated cells is shown. Anti-inflammatory activity was defined as:< 50% IL-8 production compared to H₂O₂-stimulated cells as visualized by a green line, p< 0.05. *: p< 0.05, n ≥ 3.