Table 1.
Overview of species used in this study and their respective culture conditions.
| Genus | Species | Liquid culture | Solid culture | Oxygen level |
|---|---|---|---|---|
| Actinomyces |
- A. naeslundii
- A. oris |
BHIB | NA | aerobic/microaerobic |
| Atopobium | - A. parvulum | Anaerobic BHIB + 0.2% BSA | CBA + 5% sheep blood | anaerobic |
| Gemella |
- G. haemolysans
- G. sanguinis |
BHIB + 0.2% BSA | CBA + 5% sheep blood | microaerobic |
| Micrococcus | - M. luteus | BHIB | NA | aerobic |
| Neisseria | - N. subflava | BHIB | NA | microaerobic |
| Prevotella |
- P. histicola
- P. melaninogenica - P. nigrescens - P. oris |
anaerobic BHIB | CBA + 5% sheep blood | anaerobic |
| Rothia | - R. mucilaginosa | BHIB | NA | aerobic |
| Streptococcus |
- S. salivarius
- S. sanguinis |
BHIB | NA | microaerobic |
| Veillonella | - V. atypica | anaerobic BHIB + 0.1% lactate | CBA + 5% sheep blood | anaerobic |
BHIB, Brain Heart Infusion broth (Neogen); anaerobic BHIB, BHIB supplemented with 0.1% resazurin (Sigma-Aldrich) and 5% L-cysteine (Sigma-Aldrich); BSA, Bovine Serum Albumin (Sigma-Aldrich); NA, Nutrient Agar (Neogen); CBA, Colombia Blood Agar (Neogen).