Figure 1.

Dedifferentiated gliospheres are similar to BTIC for appearance, size and heterogeneity. Microscope observations before/after dedifferentiation of T98-G (A, a), U-251MG (B, b), U-118MG (C, c), U-87MG (D, d), BTIC 25m (e) and 12m (f). Capital letters are used for adherent controls (AC) and lowercase letter for gliospheres (GS). Pictures were taken when gliospheres reached their maximum viable growth size. Scale is 10 µm. Corresponding gliospheres sizes are shown below. Areas were measured using ImageJ software for 100 gliospheres obtained from 3 independent experiments. Medians are represented in red.