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Expression of stem cells and glial marker proteins in adherent cells and in gliospheres. Typical fluorescent confocal photographs of cells immunostained to reveal Olig2 (AF488, green), Nestin (AF568, red), GFAP (AF568, red) and nuclei (DAPI, blue). Scale is 20 µm. Pictures represents BTIC 25m (A-C) and 12m (D-F), U-251MG (G-I), U-118MG (J-L), T98-G (M-O) and U-87MG (P-R), Capital letters stands for adherent cells (AC) and lowercase letters for gliospheres (GS).
