Figure 5.

The virulence of Salmonella Typhimurium retrieved from desiccated mucin droplet (0.1 wt %) impacted on a solid surface at different velocities

(A–G) Salmonella Typhimurium recovered from desiccated mucin (0.1 wt %) droplet impacted solid glass surface with or without specific velocities (5, 7, and 10 m/s) were used to infect RAW264.7 cells to determine the (A)percent phagocytosis and (B) intracellular proliferation (n = 3, N = 2). Determining the transcript-level expression of (C)sifA,(D)ssaV, and (E)phoP from intracellular Salmonella by RT-qPCR (n = 3, N = 2). Salmonella retrieved from the bacteria-laden dried droplet (mucin 0.1 wt %) impacted solid glass surface with or without velocities were used to infect C57BL/6 mice. On sixth day post-infection, the mice were sacrificed, and the bacterial burden in the (F) Liver and (G) Spleen was enumerated. Data are represented as mean ± SEM. (P) ∗< 0.05, (P) ∗∗< 0.005, (P) ∗∗∗< 0.0005, (P) ∗∗∗∗< 0.0001, ns= non-significant, (Student’s t test-unpaired).