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. 2023 Mar 25;299(5):104651. doi: 10.1016/j.jbc.2023.104651

Figure 5.

Figure 5

PRDM9 methylates nonhistone proteins.A, comparison of PRDM9 methylation of CTNNBL1 when the target Lys is substituted with an Arg (K394R). Representative of three independent experiments. Top panel depicts fluorography signal after in vitro methyltransferase reactions (see Experimental procedures), and the bottom panel shows total protein stained with Coomassie after SDS-PAGE. B, methylation of CTNNBL1 by PRDM9 coexpressed in HEK293T cells detected by immunoblot as indicated. Representative of three independent experiments.