FIGURE 5.

FLNA specifically interacts with LARP4 but not with other LARPs protein. (A) GFP-LARP5 and GFP-LARP4 were expressed in HEK293A cells and comparable expressed protein was pulled down with GST-His-FLNA R21-22 or R1-2. Bound protein was detected by western blotting using rabbit anti-GFP antibodies. (B) Sequence alignment of the potential FLNA-binding motif in different LARP family proteins. Red underline indicates LARP4 FLNA-binding motif. (C) Corresponding La modules of LARP1, LARP4 and LARP5 were expressed as GST fusion protein and incubated with 2 μM purified His-eGFP-FLNA R21-22. Bound protein was detected by western blotting using rabbit anti-GFP antibodies. 5 μg of the purified GST fusion protein was loaded to the 9% SDS-PAGE gel for CBB staining.