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. 2023 Apr 24;14:1175075. doi: 10.3389/fphys.2023.1175075

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Copyright © 2023 Hu, Bie, Lu, Ding, Guan, Geng, Ma, Hu, Fan and Shen.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Volume and quantity percentage of primary growth stage oocytes (PGO), cortical alveoli stage oocytes (CAO), and vitellogenesis stage oocytes (VTGO) of largemouth bass. (A) Volume percentage of PGO, CAO, and VTGO relative to the total volume of all oocytes. (B) Quantity percentage of PGO, CAO, and VTGO relative to the total number of all oocytes. Stage 2, supposed maturation of ovary, right after temperature increase, 25 September . Stage 4, the supposed spawning peak, spawning was observed in all treatment groups, 21 November. 18NP, 18°C overwintering water temperature, natural photoperiod group. 18CP, 18°C overwintering water temperature, controlled photoperiod group. 16CP, 16°C overwintering water temperature, controlled photoperiod group. NTNP, natural water temperature and natural photoperiod group. N = 33.