TABLE 1.
Bacterial strains and plasmids used in this study
| Plasmid or strain | Characteristics | Reference or source |
|---|---|---|
| Plasmids | ||
| pBluescript II KS | High-copy-number cloning vector; Apr | Stratagene |
| pWSK129 | Low-copy-number cloning vector; Kmr | 29 |
| pWSK29 | Low-copy-number cloning vector; Apr | 29 |
| pUCP19 | E. coli-P. aeruginosa shuttle vector; Apr | 30 |
| pPR691 | Low-copy-number cosmid vector, pSC101 replicon, and specifies resistance to Knr, Specr, and Strepr | 12 |
| pLBA577 | 4.0-kb BamHI insert in pBluescript II KS | 15 |
| pLBA589 | 7.1-kb HindIII insert in pBluescript II KS | 15 |
| pLBA591 | 1.65-kb HindIII-EcoRV fragment from pLBA589 cloned into pBluescript II KS (orfH8 complete) | This study |
| pLBA593 | 1.70-kb HindIII-BamHI fragment from pLBA589 cloned into pBluescript II KS (orfH8 complete) | This study |
| pLBA655 | Cosmid; insert containing orfH1 through to orfH31 from the Hardjobovis rfb locus | This study |
| pLBA658 | galE from the C. jejuni 81116 rfb locus in pBluescript II KS | B. Frya |
| pLBA661 | 2.04-kb BamHI-EcoRV fragment from pLBA589 cloned into pBluescript II KS (orfH10 complete) | This study |
| pLBA662 | 2.61-kb XbaI-BamHI fragment from pLBA589 cloned into pWSK129 (orfH11 complete) | This study |
| pLBA663 | 1.64-kb BamHI-HindIII fragment from pLBA577 cloned into pBluescript II SK (orfH13 complete) | This study |
| pLBA664 | 1.64-kb BamHI-HindIII fragment from pLBA577 cloned into pWSK29 (orfH13 complete) | This study |
| pLBA665 | 2.61-kb XbaI-BamHI fragment from pLBA589 cloned into pBluescript II KS (orfH11 complete) | This study |
| Bacterial strains | ||
| L. borgpertersenii | ||
| L171 | Serovar Hardjo subtype Hardjobovis | 15 |
| S. enterica serovar Typhimurium | ||
| SL696 | Wild type | 32 |
| SL761 | galE strain | 25 |
| E. coli | ||
| VCS257 | DP50 sup F[supE44 supF58 hsd53(rB mB) dapD8 lacY1 glnV44 Δ(gal-uvrB)47 tyrT58 gyrA29 tonA53 Δ(thyA57)] | Stratagene |
| DH5∝ | F−endA1 hsdR17 (mK+ rK−) supE44 thi-1 recA1 gyrA96 relA1 (φ80dlacZΔM15) | 10 |
| AB1133 | K-12 thr-1 leuB6 Δ(gpt-proA)66 hisG4 argE3 thi-1 rfbD1 lacY1 ara-14 galK2 xyl-5 mgl-51 rpsL31 supE44 | 20 |
| EC21566 | As AB1133 but rff::Tn10-66 | 20 |
| V. cholerae | ||
| O17 | Ol El Tor Ogawa | 7 |
| V1217 | Strain O17 with Knr insertion in rfbW; rough phenotype | 7 |
| P. aeruginosa | ||
| PAO1 | Band B+; serotype O5 | 4 |
| wbpM-2 | Strain PAO1 with wbpM gentamicin resistance cassette inserted at NruI; LPS B-band negative phenotype | 4 |
a
Department of Applied Biology and Biotechnology, RMIT, Australia.