Table 1.
Target peptides employed for the quantitation of SARS-CoV-2 spike (S) protein. Housekeeping proteins glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and actin (A) were also quantified to allow normalization for the number of mammalian cells that were processed. All peptides were 13C15N isotopically labeled on the C-terminal lysine or arginine and are indicated in bold font and underlined. M/z = mass/charge.
| Target Peptide | Protein | Precursor ion (m/z) | Quantification ion (m/z) | Confirmation ion (m/z) | Confirmation ion (m/z) | Collision energy (eV) |
|---|---|---|---|---|---|---|
| GVYYPDK | Spike(S1) | 421.208 (+2) | 685.319 (y5) | 359.193 (y3) | 522.256 (y4) | 15.5 |
| GVYYPDK | Spike (S1) | 425.215 (+2) | 693.332 (y5) | 367.207 (y3) | 530.270 (y4) | 15.5 |
| GIYQTSNFR | Spike (S1) | 543.272 (+2) | 624.310 (y5) | 752.369 (y6) | 915.432 (y7) | 19.2 |
| GIYQTSNFR | Spike (S1) | 548.276 (+2) | 634.318 (y5) | 762.377 (y6) | 925.440 (y7) | 19.2 |
| FLPFQQFGR | Spike (S1) | 570.303 (+2) | 879.447 (y7) | 635.326 (y5) | 782.394 (y6) | 20.0 |
| FLPFQQFGR | Spike (S1) | 575.307 (+2) | 889.455 (y7) | 645.334 (y5) | 792.403 (y6) | 20.0 |
| VTLADAGFIK | Spike (S2) | 517.797 (+2) | 721.388 (y7) | 650.351 (y6) | 535.324 (y5) | 18.4 |
| VTLADAGFIK | Spike (S2) | 521.804 (+2) | 729.402 (y7) | 658.365 (y6) | 543.338 (y5) | 18.4 |
| ASANLAATK | Spike (S2) | 423.737 (+2) | 688.399 (y7) | 617.362 (y6) | 503.319 (y5) | 15.6 |
| ASANLAATK | Spike (S2) | 427.744 (+2) | 696.413 (y7) | 625.376 (y6) | 511.333 (y5) | 15.6 |
| AAFTSGK | GAPDH | 341.181 (+2) | 539.282 (y5) | 392.214 (y4) | 610.320 (y6) | 13.1 |
| AAFTSGK | GAPDH | 345.189 (+2) | 547.297 (y5) | 400.228 (y4) | 618.334 (y6) | 13.1 |
| AITIFQER | GAPDH | 489.274 (+2) | 793.420 (y6) | 579.289 (y4) | 692.373 (y5) | 17.6 |
| AITIFQER | GAPDH | 494.278 (+2) | 803.429 (y6) | 589.297 (y4) | 702.381 (y5) | 17.6 |
| VIPELNGK | GAPDH | 435.258 (+2) | 657.356 (y6) | 560.303 (y5) | 770.440 (y7) | 16.0 |
| VIPELNGK | GAPDH | 439.265 (+2) | 665.370 (y6) | 568.318 (y5) | 778.454 (y7) | 16.0 |
| AGFAGDDAPR | Actin | 488.727 (+2) | 630.284 (y6) | 701.321 (y7) | 573.263 (y5) | 17.6 |
| AGFAGDDAPR | Actin | 493.731 (+2) | 640.292 (y6) | 711.330 (y7) | 583.271 (y5) | 17.6 |
| GYSFTTTAER | Actin | 566.767 (+2) | 678.342 (y6) | 577.294 (y5) | 825.410 (y7) | 19.9 |
| GYSFTTTAER | Actin | 571.771 (+2) | 688.350 (y6) | 587.302 (y5) | 835.418 (y7) | 19.9 |
| IIAPPER | Actin | 398.239 (+2) | 569.304 (y5) | 498.267 (y4) | 401.214 (y3) | 14.9 |
| IIAPPER | Actin | 403.243 (+2) | 579.312 (y5) | 508.275 (y4) | 411.223 (y3) | 14.9 |