Table 2.
Cells were transfected in triplicate with expired commercial vaccine material, lysed, and analyzed by IDMS. Target peptides were were used to quantify spike, GAPDH, and actin. The average and % RSD were calculated by considering each peptide result of each replicate as an independent data point. The % RSD for each protein was less than 15.
| Spike (fmol/cell pellet) | ASANLAATK | GVYYPDK | GIYQTSNFR | FLPFQQFGR | VTLADAGFIK | Average | Std. Dev. | % RSD |
|---|---|---|---|---|---|---|---|---|
| 50 µg vaccine prep 1 | 5.7 | 5.2 | 4.7 | 6.5 | 5.6 | 5.2 | 0.8 | 14.5 |
| 50 µg vaccine prep 2 | 4.7 | 4.3 | 4 | 5.2 | 4.4 | |||
| 50 µg vaccine prep 3 | 5.6 | 5.4 | 5.1 | 6.7 | 5.5 | |||
| GAPDH (pmol/cell pellet) | AAFTSGK | VIPELNGK | AITIFQER | |||||
| 50 µg vaccine prep 1 | 39.7 | 54.1 | 43.6 | 45.1 | 5.5 | 12.2 | ||
| 50 µg vaccine prep 2 | 37.5 | 48.9 | 40.7 | |||||
| 50 µg vaccine prep 3 | 44.8 | 51.2 | 45.4 | |||||
| Actin (pmol/cell pellet) | AGFAGDDAPR | IIAPPER | GYSFTTTAER | |||||
| 50 µg vaccine prep 1 | 297.8 | 255.6 | 300.5 | 294.8 | 25.4 | 8.6 | ||
| 50 µg vaccine prep 2 | 322 | 281.8 | 310.5 | |||||
| 50 µg vaccine prep 3 | 328 | 258.3 | 298.4 |