TABLE 4.
Complementation of biofilm formation in different biofilm-negative mutant classes by icaADBC expressed by a xylose-dependent promoter
| Strain (class)a | Biofilm formation (OD570)b on TSBOxoid:
|
|
|---|---|---|
| Without xylose | With 4% xylose | |
| 1457 (wt) | 2.10 | 2.30 |
| M13 (I) | 0.03 | 0.02 |
| M13/pTXicaADBC | 0.04 | 2.20 |
| M12 (II) | 0.01 | 0.02 |
| M12/pTXicaADBC | 0.01 | 1.03 |
| M15 (III) | 0.02 | 0.02 |
| M15/pTXicaADBC | 0.05 | 1.06 |
| M17 (IV) | 0.07 | 0.06 |
| M17/pTXicaADBC | 0.09 | 2.50 |
a
wt, wild type.
b
Biofilm formation was assessed as described in Materials and Methods using TSBOxoid supplemented or not supplemented with 4% xylose as indicated.