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. 2023 Apr 24;13:1183442. doi: 10.3389/fonc.2023.1183442

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Copyright © 2023 Choi, Ju, Kang, Seo, Kim, Miyoshi, Yeo and Park

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Comparison of glycosylation between EVs isolated via two different approaches. (A) Schematic of the EV isolation methods. SC, serial centrifugation. EQ, ExoQuick. (B) Electron microscopy (EM) assessment of the size and morphology of isolated EVs. Representative images are shown. Scale bar = 20 nm. (C) Nanoparticle tracking analysis for the quantitation of EV size. (D) Western blotting using antibodies against EV components. n = 3. (E) Lectin blot assessing the glycosylation profiles of isolated EVs. The blotted membrane was stained with Coomassie Brilliant Blue, n = 3.