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. 2023 Apr 24;14:1176748. doi: 10.3389/fphys.2023.1176748

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Copyright © 2023 Schubert, Gaynullina, Shvetsova and Tarasova.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Control for similarity of initial contractility. As an example, the protocol of an experiment is shown in which 4 groups of blood vessels will be studied in myograph units 1–4. After the initial distension (i.e., normalization) and the starting procedure (i.e., viability testing) the effect of the contractile agonist methoxamine is tested by determining its concentration-response relationship (CRR) (protocol scheme shown in the left panel). As shown in the right panel, vessel tension in the 4 different experimental groups (CONTROL1—CONTROL4) were not different (n = 7; p = 0.94 based on the area under the concentration-response relationships). Reproduced and modified with permission from (Schmid et al., 2018).