Figure 2.
Functional diversity and dynamic regulation processes of injured cells during AKI
(A) UMAP plots showing the injured mixed cell cluster distribution at each group with ischemia time series.
(B) Bar plots display the percentage of cells of four subclusters in different groups.
(C) Violin plots show the expression of selected markers (Lcn2 and Havcr1) for renal injury in different groups.
(D) Pseudotime trajectory shows the transition from normal to injured stages colored by the time course. Numbers 1 and 2 represent the branch points of different cell states.
(E) Heatmap showing differentially expressed genes (DEGs) alone with the pseudotime as in (D), catalogs into three gene modules.
(F) Gene ontology (GO) terms enriched for each gene module as in (E).
(G) Boxplots of the expression levels of selected functions across four groups in each cluster. Groups are shown in different colors. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001, ns. for not significant, Mann-Whitney-Wilcoxon test.