Figure 3.
Characterization of inflammatory cells response to acute kidney injury
(A) UMAP plot of clusters of macrophages and neutrophils colored as in Figure 1.
(B) Bar plots showing the distribution of each cluster in different groups.
(C) Dot plot displaying expression patterns of marker genes in each cluster.
(D) The enriched GO terms of each macrophage subcluster.
(E) Dot plot showing the scores of GO biological process (GOBP) terms compared between AKI-24 with Sham group among three subclusters of macrophages.
(F) Boxplots of the expression levels of inflammatory response across four groups in each macrophage cluster. Groups are shown in different colors. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001, ns. for not significant, Mann-Whitney-Wilcoxon test.
(G) Representative images of Ly6g and Mmp9 co-staining in mouse kidney sections from sham and AKI subgroups. n = 5 in each group. ∗∗p < 0.01, ∗∗∗p < 0.001, One-way ANOVA. Scale bar, 100 μm. Data are presented as mean ± SEM.
(H) Boxplots of expression levels of three GOBP terms in neutrophils across four groups. Groups are shown in different colors. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001, ns. for not significant, Mann-Whitney-Wilcoxon test.