Fig. 6.

Fr decreased the ability of endometrial cells to adhere to mesothelial cells. 12Z cells were stained with CMFDA after 24 h of Fr treatment and then gently transferred onto a red fluorescent Met-5A cell monolayer. After 90 min with gentle shaking at 20 rpm, unbound 12Z cells were removed, and cells were then visualized using a fluorescent microscope (200 × magnification) (A). The number of 12Z cells bound to Met-5A cells was manually counted in four randomly chosen areas for statistical analysis (B). Data are representative of one of five independent experiments and values are expressed in mean ± SEM. Statistical analysis was conducted using a Dunnet one-way ANOVA (***; p < 0.001).