Fig. 7.

RNA-sequencing analysis suggests possible mode of action (MoA) underlying anti-endometriosis effect of Fr. The 12Z cells were treated with Fr (70 μg/ml) for 12 h. The RNA-sequencing dataset supplied by commercial service was applied to GSEA. The results from hallmark and KEGG analysis were visualized by bubble plot. The major pathways related with endometriosis was indicated by red (upregulated) or blue (downregulated) characters (A, B). The data from GO analysis was described by Cytoscape with enrichment map visualization. The cutoff value was set as p > 0.001 and q > 0.5 (C). The DEG analysis visualized as log2 fold change and -log10 p-value. Genes that were noticeably up- or down-regulated were denoted by red or blue characters, respectively. The genes participating in endometriosis-related pathways, as shown in the A and B panels, were indicated by a symbol for each gene (D). Heatmap analysis was used to display the relative expression levels of genes connected to endometriosis-related pathways (E). A graphical scheme was used to outline the potential mechanisms of action (MoA) underlying the relieving effects of Fr on endometriosis (F).