TABLE 2.
Intestinal cell proliferation in fasted, fasted-refed, or continuously fed sheep
| Parametera | Feeding groupb | Mean ± SE results in:
|
|||||
|---|---|---|---|---|---|---|---|
| Ileum | Cecum | Proximal | Central | Distal | All locations | ||
| Labeled cells (no. of cells/crypt) | Fasted | 40.1 ± 11.8 | 67.6 ± 7.2 | 45.8 ± 10.9 | 22.9 ± 5.0 | 26.3 ± 8.5 | 40.5 ± 5.5 |
| Fasted-refed | 51.2 ± 13.8 | 55.2 ± 3.0 | 63.2 ± 12.3 | 38.7 ± 9.1 | 62.9 ± 9.2c | 54.2 ± 4.5 | |
| Continuously fed | 66.4 ± 2.2 | 65.9 ± 16.1 | 52.3 ± 4.5e | 40.3 ± 14.4 | 33.6 ± 6.1 | 51.7 ± 5.6 | |
| Crypt height (no. of cells/crypt column) | Fasted | 79.6 ± 10.0 | 73.9 ± 6.0 | 64.8 ± 10.9 | 70.2 ± 6.8 | 55.5 ± 2.7 | 68.8 ± 3.7 |
| Fasted-refed | 70.2 ± 1.2 | 61.8 ± 2.3 | 61.4 ± 8.1 | 68.2 ± 4.5 | 58.0 ± 3.5 | 63.9 ± 2.1 | |
| Continuously fed | 88.7 ± 20.6 | 77.2 ± 13.7 | 70.4 ± 8.4 | 84.1 ± 7.4 | 65.1 ± 4.0 | 77.1 ± 5.2 | |
| Proliferation index (%) | Fasted | 26.5 ± 8.3 | 45.5 ± 1.2 | 34.3 ± 3.0 | 16.0 ± 2.6 | 23.5 ± 7.3 | 29.2 ± 3.4 |
| Fasted-refed | 36.8 ± 10.2 | 44.7 ± 1.8 | 50.7 ± 5.5 | 27.9 ± 5.6 | 53.9 ± 6.0c | 42.8 ± 3.5d | |
| Continuously fed | 40.9 ± 7.5 | 42.0 ± 3.9 | 41.7 ± 0.4e | 24.8 ± 8.9 | 25.6 ± 3.5 | 34.5 ± 3.2 | |
a
Parameters are as defined in Table 1.
b
Fasted animals had feed and water withheld for 48 h, refed animals were fasted and had alfalfa hay for 24 h, and fed animals had continuous daily feeding.
c
Refed values were higher than fasted or fed values (P = 0.04 for labeled cells and P = 0.017 for proliferation index as determined by one-way ANOVA).
d
Significant effect of refeeding over all locations (P = 0.024; split-plot ANOVA).
e
n = 2; in all other cases, n = 3.