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. 2023 May 7;13(5):e1260. doi: 10.1002/ctm2.1260

FIGURE 3.

FIGURE 3

Cytoplasmic localisation of BATF2 is mediated by chromosome region maintenance 1 (CRM1)‐dependent nuclear export. (A) The heatmap of label‐free liquid chromatography–mass spectrometry/mass spectrometry (LC–MS/MS) proteomic analysis of the co‐immunoprecipitated proteins in CCD 841 CoN and HCT116 cells by using immunoglobulin G (IgG) and BATF2 antibodies separately. (B) Bioinformatic prediction of the binding between BATF2 and CRM1 by using a ZDOCK method. Important amino acids of CRM1 are highlighted in pink, including GLU383 to GLU364, and red, including LEU467 to SER450. Other structures of CRM1 are highlighted in yellow, and structures of BATF2 are highlighted in blue. (C) Co‐immunoprecipitation (co‐IP) analysis of the binding between BATF2 and CRM1 in HCT116 cells. (D) Western blot analysis of the nuclear and cytoplasmic BATF2 expression in HCT116 cells incubated with control or a CRM1 inhibitor, leptomycin B (LMB). (E–G) Western blot and immunofluorescence analysis of the nuclear BATF2 expression in HCT116 cells transfected with control siRNAs (siControl) or siRNAs against CRM1 (siCRM1). Scale bar: 10 µm.