Figure 1.

Experimental protocol for spheroid formation, culture and exposure to drugs

EwS cells were suspended in culture medium and introduced to the culture microfluidic chip, where 3D spheroids were formed and cultured. In parallel, droplet drug libraries were created in a different microfluidic chip. Such drug droplets were introduced as secondary droplets to the culture chip, where they were fused to primary droplets containing spheroids, challenging in this manner the spheroids with the drugs. Subsequent systematic imaging and image analysis resulted in relevant time-dependent viability information. Scale bar represents 100 μm.