Figure 5.

Simultaneously combined drugs on EwS spheroids

(A) Two-color fluorescence image of each well after the addition of the secondary droplet allows a measurement of the drug concentrations in each well. Right: Representation of each condition on a 2D parameter space. Each dot represents one spheroid tested at a given combination. Controls with one drug or no drug are also included in this visualization. The experiment was performed using 4 chips, containing a total of 320 droplets.

(B) Timeline of the assay. Cells are seeded and 24 h later, when EwS spheroids are formed, they are exposed to both drugs at specific concentrations and imaged every 24 h.

(C)EwS spheroids viability heat maps over 6 days. The color of each square represents the averaged viability in the corresponding concentration range.