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. 2023 Apr 13;26(5):106664. doi: 10.1016/j.isci.2023.106664

Figure 2.

Figure 2

Tissue-specific deletion of SNAP-23 results in no significant changes to fEPSP measures of hippocampal CA1 pyramidal neurons

The Schaffer collateral axonal fibers were stimulated twice of 50 ms apart and stimulating intensities varied successively from 10 to 150 μA. The resulting local fEPSP from CA1 pyramidal cell apical dendrites were recorded.

(A) Sample traces from control and SNAP-23 cKO mice of dendritic fEPSP. Stimulation artifact truncated for illustrator purposes.

(B) fEPSP amplitudes and (C) fEPSP slope were plotted against the presynaptic fiber volley amplitude for control (first stimulation, black; second stimulation, red), and SNAP-23 cKO (first stimulation, green; second stimulation, blue) mice. No difference in amplitude observed between groups for first stimulation [mixed ANOVA: F(1,47) = 0.354, p = 0.555] or second stimulation [F(1,47) = 0.085, p = 0.772]. No difference in slope observed between groups for first stimulation [F(1,47) = 0.001, p = 0.980] or second stimulation [F(1,47) = 0.007, p = 0.935].

(D and E) Paired-pulse ratios of amplitudes (D) or slopes (E) were plotted against presynaptic fiber volley amplitudes. No significant changes between SNAP-23 cKO and controls were observed for paired pulse ration amplitude [F(1,47) = 1.821, p = 0.185] or slope [F(1,10) = 0.030, p = 0.864]. For all measures, control n = 6 mice, 28 slices; SNAP-23 cKO n = 5 mice, 21 slices. Error bars indicate SEM.