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. 2023 Apr 13;26(5):106664. doi: 10.1016/j.isci.2023.106664

Figure 6.

Figure 6

CA1-specific SNAP-23 cKO results in defective Schaffer collateral-CA1 LTP and significantly impairs Morris water maze performance. See also Figure S4

(A) Representative recordings of fEPSP of control and SNAP-23 cKO CA1 at baseline (1), immediately after LTP induction (2), and 50 min after LTP induction (3); black traces control, red traces SNAP-23 cKO. Each illustrated trace was averaged from four consecutively evoked responses.

(B) Average fEPSP slope normalized against baseline. Data were collected from 9 slices of KO mice and 8 slices of control mice.

(C) Normalized fEPSP slope for control and SNAP-23 cKO at times 2 (immediately after LTP induction) and 3 (50 min after LTP induction). Error bars indicate SEM (independent t-test, t(6) = 0.617, p = 0.547 for timepoint 2, t(15) = 3.835, p = 0.002 for timepoint 3).

(D) Learning curves for mice to find the visible platform (days 1–3), hidden platform (days 4–12) and hidden platform during reversal training (days 13–15). Animals were tried 4 times a day, with randomized entry points to the pool. Latency to find the hidden platform was significantly increased in the SNAP-23 cKO mice relative to the controls [mixed ANOVA; days 4–12: F(1,10) = 101.9, p < 0.001; days 13–15: F(1,10) = 6.924, p = 0.025], however, SNAP-23 cKO mice still demonstrate some degree of learning [F(8,10) = 14.752, p < 0.001].

(E) During Probe Test, platform was removed from the pool and % of time spent in quadrant where platform was previously located was calculated from total recording time of 60 s, SNAP-23 cKO mice spent less time in the correct quadrant during the probe test [F(1,10) = 55.420, p < 0.001]. Error bars indicate SEM.