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. 2023 May 8;222(6):e202210059. doi: 10.1083/jcb.202210059

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© 2023 Amm et al.

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Figure S5. — Deletion of NDC1 causes alterations in NE morphology. (A) Fluorescence microscopy of mitotic yeast cells, as indicated, expressing the GFP-tagged nucleoporin ScNup49 as NPC marker. White arrows mark GFP-ScNup49 as part of cytoplasmic NE/ER expansions. Hoechst 33258 serves as a chromatin stain. Bars, 5 µm. (B) Fluorescence microscopy of mitotic yeast cells, as indicated, coexpressing GFP-tagged ScPus1 as nucleoplasm marker protein and the mRFP-tagged nucleolar marker protein ScNop1. White arrows mark nuclear expansions not overlapping with nucleoli. Red arrows mark Nop1-positive nuclear flares. Hoechst 33258 serves as a chromatin stain. Bars, 5 µm. (C and D) Electron micrographs of ndc1Δ nup53Δ nup59Δ (C) and ndc1Δ pom34Δ (D) cells both showing abnormalities of the nuclear morphology. Bars, 1 µm.