Figure 3.

Contribution of spike, membrane and nucleocapsid peptide pools to the SARS-CoV-2 specific T-cell response in patients with mild, moderate and COVID-19. Absolute (A), and relative (B) proportion presented as mean percentage of spike (S and S1), membrane (M) and nucleocapsid (N) peptide pools of total SARS-CoV-2 specific T-cell reactivity (sum SFC of S, S1, M and N). Data derived from sera that were collected per month after symptom onset, measured by ELISpot in patients with mild (n=58), moderate (n=90) and severe COVID-19 (n=100). *T-cell responses from mild disease group were obtained from thawn PBMCs, whereas the T-cell responses from hospitalized patient with moderate or severe disease were measured in fresh PBMCs. Comparability was ensured based on the presence of a similar mitogen response between both sample groups.