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. 2023 Apr 24;14:1146496. doi: 10.3389/fmicb.2023.1146496

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Copyright © 2023 Camus, Espinosa, Zapater Baras, Singh, Quenech’Du, Vickridge, Modesti, Barre and Espéli.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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RecN’s influence of sister chromatid interactions differs according to the DNA lesion. (A) CFU of WT and recN mutant in the presence of MMC (5 μg/mL) or BLM (1 μg/mL). Results are expressed as percentage of survival compared to the untreated condition. (B) Induction of RecN in the presence of MMC (5 μg/mL) or BLM (1 μg/mL) measured by western blot. (C) SOS and arabinose promoters expression in the presence of MMC and BLM. Both drugs were added at the time point 90 min. (C) Hi-SC2 profile of sister chromatid interactions in the WT and recN mutant in the presence or absence of MMC or BLM. (D) Hi-SC2 inter sisters recombination frequency measures of WT and recN strains immediately after a 20 min of MMC or BLM treatment and recovering of their injuries for 50 min before measure. Anova statistical test *<0.05, **<0.005, ***<0.0005, ****<0.00005.