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. 2000 Jul;68(7):3830–3839. doi: 10.1128/iai.68.7.3830-3839.2000

TABLE 2.

Dose dependency of the predictive power of salivary, urinary, and serum antibodies for the antibody responses at remote mucosal surfacesa

Antibody response OVA dose (mg)
Antibody response OVA dose (mg)
0.2
2
20
0.2
2
20
Titer ratiob rc Titer ratio r Titer ratio r Titer ratio r Titer ratio r Titer ratio r
Absolute salivary IgA in relation to absolute: Relative salivary IgA in relation to relative:
 Urinary IgA 9.6 × 10−2 1.00** — −0.03 — −0.05  Urinary IgA 7.0 × 10−3 0.84* — 0.74 — 0.61
 Serum IgA 5.1 × 10−4 0.97** — 0.01 — 0.39  Serum IgA 3.6 × 10−3 0.93* — 0.67 — 0.79
 Serum IgG 2.2 × 10−5 0.98** — −0.41 — 0.57  Serum IgGd 1.4 × 10−5 1.00** — −0.56 — 0.06
 Duodenal IgA 2.8 × 10−4 0.99** — −0.61 — −0.23  Duodenal IgA 3.2 × 10−3 0.99** — 0.09 — 0.05
 Jejunal IgA 1.3 × 10−4 0.95* — −0.61 — 0.62  Jejunal IgA 1.1 × 10−3 0.92* — 0.27 2.7 × 10−1 0.92*
 Ileal IgA 1.6 × 10−4 0.97** — −0.25 — −0.03  Ileal IgA 2.0 × 10−3 0.97** — 0.44 — 0.66
 Rectal IgA 1.3 × 10−3 0.97** — −0.15 — 0.26  Rectal IgA 4.0 × 10−3 0.92* — 0.69 — 0.69
 Vaginal IgA — 0.24 — 0.02 — 0.50  Vaginal IgA — 0.36 — 0.49 — 0.29
 Fecal IgA — 0.54 — 0.01 — 0.23  Fecal IgA — 0.52 — 0.23 — 0.69
Absolute urinary IgA in relation to absolute: Relative urinary IgA in relation to relative:
 Salivary IgA 1.0 × 101 1.00** — −0.03 — −0.05  Salivary IgA 1.4 × 102 0.84* — 0.74 — 0.61
 Serum IgA 5.3 × 10−3 0.97** 1.4 × 10−2 0.95* — 0.00  Serum IgA 5.1 × 10−1 0.97** — 0.69 — 0.27
 Serum IgG 2.3 × 10−4 0.97** — 0.34 — 0.34  Serum IgGd 2.0 × 10−3 0.87* — −0.01 — 0.34
 Duodenal IgA 2.9 × 10−3 0.99** — 0.53 — −0.30  Duodenal IgA 4.5 × 10−1 0.84* — 0.13 — 0.24
 Jejunal IgA 1.3 × 10−3 0.95* — 0.53 — −0.35  Jejunal IgA — 0.59 — 0.40 — 0.48
 Ileal IgA 1.7 × 10−3 0.98** 4.4 × 10−3 0.96** — 0.60  Ileal IgA 2.9 × 10−1 0.88* — 0.71 — 0.25
 Rectal IgA 1.3 × 10−2 0.98** — 0.75 — 0.41  Rectal IgA — 0.59 — 0.74 — 0.55
 Vaginal IgA — 0.23 2.1 × 10−2 0.86* — 0.14  Vaginal IgA — 0.80 — 0.59 — 0.28
 Fecal IgA — 0.57 2.2 × 10−3 0.94* — 0.23  Fecal IgA 2.1 0.82* — 0.70 — 0.27
Absolute serum IgA in relation to absolute: Relative serum IgA in relation to relative:
 Salivary IgA 2.0 × 103 0.97** — 0.01 — 0.39  Salivary IgA 2.8 × 102 0.93* — 0.67 — 0.79
 Urinary IgA 1.9 × 102 0.97** 7.2 × 101 0.95* — 0.00  Urinary IgA 2.0 0.97** — 0.69 — 0.27
 Serum IgG 4.4 × 10−2 0.99** — 0.25 — −0.18  Serum IgGd 4.0 × 10−3 0.95* — 0.02 — −0.15
 Duodenal IgA 5.6 × 10−1 0.95* — 0.66 — −0.20  Duodenal IgA 8.9 × 10−1 0.94* — 0.50 — −0.17
 Jejunal IgA 2.5 × 10−1 0.89* — 0.66 — 0.63  Jejunal IgA — 0.76 — 0.80 — 0.73
 Ileal IgA 3.2 × 10−1 0.95* 3.2 × 10−1 0.86* — 0.60  Ileal IgA 5.7 × 10−1 0.97** 2.3 × 10−1 0.85* — 0.45
 Rectal IgA 2.5 0.95* — 0.78 — 0.31  Rectal IgA — 0.76 7.2 × 10−2 0.97** — 0.71
 Vaginal IgA — 0.45 — 0.79 — −0.12  Vaginal IgA — 0.63 — 0.59 — 0.37
 Fecal IgA — 0.67 1.6 × 10−1 0.95* — 0.43  Fecal IgA — 0.76 — 0.69 — 0.25
Absolute serum IgG in relation to absolute: Absolute serum IgG in relation to relative:
 Salivary IgA 4.5 × 104 0.98** — −0.41 — 0.57  Salivary IgA 7.0 × 104 1.00** — −0.56 — 0.06
 Urinary IgA 4.3 × 103 0.97** — 0.34 — 0.34  Urinary IgA 4.9 × 102 0.87** — −0.01 — 0.34
 Serum IgA 2.3 × 101 0.99** — 0.25 — −0.18  Serum IgA 2.5 × 102 0.95* — 0.02 — −0.15
 Duodenal IgA 1.3 × 101 0.95* — 0.13 — −0.67  Duodenal IgA 2.2 × 102 0.99** — −0.14 — −0.69
 Jejunal IgA 5.7 0.88* — 0.13 — −0.29  Jejunal IgA 7.5 × 101 0.89* — 0.14 — 0.17
 Ileal IgA 7.1 0.93* — 0.53 — −0.30  Ileal IgA 1.4 × 102 0.97** — 0.45 — −0.44
 Rectal IgA 5.7 × 101 0.93* — 0.31 — −0.22  Rectal IgA 2.8 × 102 0.90* — 0.09 — −0.33
 Vaginal IgA — 0.45 — 0.65 2.8 × 101 0.93*  Vaginal IgA — 0.42 — 0.11 — 0.80
 Fecal IgA — 0.62 — 0.49 — −0.34  Fecal IgA — 0.56 — 0.40 — −0.12
a

Linear body fluid-to-site correlations of anti-OVA antibody titers after four intragastric immunizations with OVA (amounts indicated) plus CT adjuvant. Significant correlations between anti-OVA responses are highlighted by bold characters. 

b

Titer ratios were calculated from the respective geometric group means (six samples per group). The numbers show the mean antibody titer of the respective body fluid divided by the mean antibody titer of the mucosal site or body fluid indicated in the table. —, no titer ratios were calculated for nonsignificant correlations. 

c

r, Pearson's correlation coefficient. Asterisks indicate the significance of the correlations determined after Fisher's r-to-z transformation of the correlation coefficient: ∗, P < 0.05; ∗∗, P < 0.001. 

d

Absolute serum IgG titers were used for this analysis.